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KMID : 0357919990330111047
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Korean Journal of Pathology
1999 Volume.33 No. 11 p.1047 ~ p.1054
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Type IV Collagen mRNA Expression in Human Membranous Nephropathy
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Kim Tae-Sook
Kim Jung-Yeon
Hong Hye-Kyoung
Lee Hyun-Soon
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Abstract
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Human membranous nephropathy (MN) is morphologically characterized by subepithelial immune complex deposits and progressive thickening of glomerular basement membranes (GBM). Studies have suggested that the enhanced secretion of classical and novel type IV collagen chains in MN contributes to spike formation and the novel type IV collagen chain is particularly related to thickening of GBM. It is unclear whether the increased accumulation of extracellular matrix (ECM) proteins in GBM is due to the increased mRNA expression for type IV collagen in glomerular visceral epithelial cells (GECs). To answer this question, we analyzed seven renal biopsies of patients with idiopathic MN using in situ hybridization. In MN, the number of GECs expressing mRNA for α1(IV) collagen was 2.82 1.80/glomerular cross section (gcs), and the number expressing mRNA for α4(IV) collagen was 8.42 2.85/gcs. The number of GECs expressing mRNA for α4(IV) collagen was significantly larger than that of α1(IV) collagen mRNA. The expression of mRNA for these ECM proteins in normal controls was negligible. These results suggest that subepithelial immune complexes stimulate the gene expression of α1(IV) collagen and α4(IV) collagen in glomerular GECs which, in turn, increase the secretion of ECM proteins and contribute to the thickening of GBM in MN.
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KEYWORD
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Membranous nephropathy, α1(IV) collagen, α4(IV) collagen, mRNA, in situ hybridization
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